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Expression of pancreatic secretory trypsin inhibitor gene in neoplastic tissues
Author(s) -
Tomita Naohiro,
Horii Akira,
Yamamoto Tatsuo,
Ogawa Michio,
Mori Takesada,
Matsubara Kenichi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81141-9
Subject(s) - psti , microbiology and biotechnology , complementary dna , northern blot , biology , trypsin inhibitor , southern blot , gene expression , gene , cdna library , trypsin , biochemistry , enzyme , restriction enzyme
Expression of the human pancreatic secretory trypsin inhibitor (PSTI) gene was examined in 24 cases of neoplastic tissues by Northern blot analyses. In three cases of lung adenocarcinoma and one case of sigmoid colon polyp, we detected transcripts which hybridized to the human PSTI cDNA probe. cDNA libraries were constructed using mRNAs of the two PSTI‐positive tumor tissues. Two PSTI cDNA clones were obtained from each sample. Sequencing analyses showed that they were completely identical with that of pancreatic PSTI cDNA which had been reported [(1985) Biochem. Biophys. Res. Commun. 132, 605–612]. Southern blot analyses showed that the elevated expression of PSTI in neoplastic tissues was accompanied by neither PSTI gene amplification nor rearrangements.