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Epidermal growth factor (EGF), tumor promoter 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium and stimulate arachidonic acid release and PGE 2 /6‐keto PGF 1α production in cultured porcine thyroid cells
Author(s) -
Takasu Nobuyuki,
Yamada Takashi,
Shimizu Yoshifusa
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81128-6
Subject(s) - 12 o tetradecanoylphorbol 13 acetate , arachidonic acid , calcium , epidermal growth factor , ionophore , endocrinology , chemistry , medicine , tetradecanoylphorbol acetate , second messenger system , stimulation , biochemistry , biology , protein kinase c , phosphorylation , receptor , enzyme , phorbol ester
Epidermal growth factor (EGF), 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium ([Ca 2+ ] i ) and stimulate arachidonic acid release and production of PGE 2 and 6‐keto PGF 1α an end metabolite of PGI 2 , in cultured porcine thyroid cells. Addition of EGF, TPA or A23187 to the cells loaded with fura‐2, a fluorescent Ca 2+ indicator, causes an immediate increase in [Ca 2+ ] i which is the earliest event after mitogen stimulation. This [Ca 2+ ] i response occurs immediately, reaching a maximum within several seconds. EGF, TPA and A23187 stimulate arachidonic acid release and PGE 2 and 6‐keto PGF 1α production; the maximum effects are obtained after 2–4 h incubation. EGF, TPA and A23187 increase [Ca 2+ ] i and then stimulate arachidonic acid release and PG production.