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Murine interleukin‐1 receptor: Differences in binding properties between fibroblastic and thymoma cells and evidence for a two‐chain receptor model
Author(s) -
Bird T.A.,
Gearing A.J.H.,
Saklatvala J.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81124-9
Subject(s) - receptor , thymoma , microbiology and biotechnology , biology , cell culture , endocrinology , interleukin 2 , interleukin , cytokine , medicine , chemistry , immunology , biochemistry , genetics
The concentration of porcine interleukin‐1β (pIL1β) required to elicit half‐maximal IL2 production from NOB‐1, a subline of murine thymoma EL4, was 100‐fold greater than for p1Lα. In contrast, similar doses of each type of IL1 stimulated increased lactate production by Balb/C 3T3 fibroblasts. Receptor‐bound 125 I‐IL1α was displaced with equal efficiency by both unlabelled forms from 3T3 cells, but a 20‐fold lower affinity for p1L1β was observed using NOB‐1. Crosslinking experiments suggested that the IL1 receptors on each line consisted of two polypeptides of 80 and 100 kDa. The results provide the first evidence for a multiple‐component IL1 receptor within which IL1α and IL1β may bind at different loci, and suggest the receptors may have evolved differently in the two lines.