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Concurrent measurements of the inhibition of ATP synthesis and alanine transport in intact cells of Rhodobacter capsulatus
Author(s) -
Crielaard W.,
Elferink M.G.L.,
Hellingwerf K.J.,
Konings W.N.,
Myatt J.F.,
Jackson J.B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81121-3
Subject(s) - rhodobacter , antimycin a , atp synthase , alanine , electron transport chain , chemiosmosis , biochemistry , adenosine triphosphate , chemistry , biophysics , biology , mutant , enzyme , amino acid , gene
Myxothiazol, antimycin A and carboxylcyanide‐ m ‐chlorophenylhydrazone (CCCP) all had a more pronounced inhibitory effect on the rate of ATP synthesis than on the rate of alanine transport during illumination of intact cells of Rhodobacter capsulatus . CCCP had the most potent effect and antimycin A the least. The data suggest that the energy‐input requirement for alanine transport is lower than that for ATP synthesis. A new test for direct interactions between the electron transport system and the ATP synthase and alanine transporter is applied to the data. Subject to reservations about the accuracy of rate measurement in intact bacterial cells and about the specificity of inhibition, the test reveals that the proton motive force is not the sole determinant of the rate of ATP synthesis and the rate of alanine transport.