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Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC
Author(s) -
Mann Karlheinz,
Deutzmann Rainer,
Paulsson Mats,
Timpl Rupert
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81040-2
Subject(s) - osteonectin , edman degradation , basement membrane , chemistry , peptide sequence , biochemistry , biology , microbiology and biotechnology , gene , enzyme , osteocalcin , alkaline phosphatase
Up to 80% of the calcium‐binding protein BM‐40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.