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Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium
Author(s) -
Mallea L.E.,
Machado A.J.,
Navaroli F.,
Rommerts F.F.G.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81035-9
Subject(s) - medicine , endocrinology , epidermal growth factor , sertoli cell , aromatase , follicle stimulating hormone , protein kinase c , biology , chemistry , hormone , luteinizing hormone , signal transduction , microbiology and biotechnology , receptor , spermatogenesis , cancer , breast cancer
Aromatization of testosterone by cultured Sertoli cells isolated from immature rats was stimulated more than 7‐fold by follicle stimulating hormone (FSH) or dcAMP. The effects of FSH and dcAMP could be partly inhibited by epidermal growth factor (EGF) in a dose‐dependent manner (ID 50 0.5 nM). The phorbol ester 4β‐phorbol‐12‐myristate‐13‐acetate (PMA) could also inhibit aromatase activity in a fashion similar to EGF. When 3 mM EGTA was present in the culture medium, the inhibitory effect of EGF was abolished but the stimulatory effect of FSH or dcAMP was magnified. These results suggest that EGF exerts a negative control on aromatase via calcium and protein kinase C. The abolishment of the inhibitory effect of EGF and the enhancement of the stimulatory effect of FSH or dcAMP by a calcium deficiency may be an indication that growth factors produced by Sertoli cells negatively control FSH‐induced responses in an autocrine fashion.