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Interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca 2+
Author(s) -
Shivaji Sisinthy
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81026-8
Subject(s) - chlortetracycline , fluorescence , chemistry , chelation , binding site , salt (chemistry) , biochemistry , inorganic chemistry , antibiotics , organic chemistry , physics , quantum mechanics
The interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca 2+ , was studied. The results indicate that seminalplasmin binds to chlortetracycline. The binding is not influenced by salt. Both Ca 2+ and seminalplasmin probably bind to the same site on chlortetracycline. Seminalplasmin also reduced the Tb 3+ ‐associated fluorescence of bovine spermatozoal plasma membrane. These results are discussed in relation to the inhibitory effect of seminalplasmin on the uptake of Ca 2+ in bovine spermatozoa.