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Arginine‐vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells
Author(s) -
Nielsen Jane R.,
Hansen Harald S.,
Jensen Benny
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81025-6
Subject(s) - phosphatidic acid , vasopressin , endocrinology , medicine , arginine , arachidonic acid , agonist , stimulation , receptor , chemistry , leydig cell , diacylglycerol kinase , vasopressin receptor , biology , antagonist , amino acid , phospholipid , biochemistry , signal transduction , hormone , protein kinase c , membrane , luteinizing hormone , enzyme
Arginine‐vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [ 14 C]arachidonic acid‐prelabelled rat Leydig cells. After addition of 10 t̄6 M AVP [ 14 C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose‐dependent (10 −11 −10 −6 M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V 1 antagonist dPTyr(Me)AVP inhibited in a dose‐dependent manner the AVP‐stimulated accumulation of PA. The V 2 agonist dPVDAVP was without effect. The present results suggest that AVP binds to V 1 receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP‐stimulated PA formation is an indication of phosphoinositide turnover.