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Transcriptional enhancer activity in the variable tandem repeat DNA sequence downstream of the human Ha‐ ras 1 gene
Author(s) -
Spandidos Demetrios A,
Holmes Lesley
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81014-1
Subject(s) - enhancer , gene , tandem repeat , dna , sequence (biology) , genetics , biology , downstream (manufacturing) , microbiology and biotechnology , tandem , computational biology , chemistry , gene expression , genome , operations management , materials science , economics , composite material
A short term transfection technique using the chloramphenicol acetyltransferase gene as an assay system has been employed to examine the presence of transcriptional regulatory sequences within the variable tandem repeat (VTR) DNA sequence located downstream of the human Ha‐ ras 1 gene. Here we find that the VTR sequences possess an endogenous enhancer activity of both the normal and the T24 mutant Ha‐ ras 1 gene.