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Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription
Author(s) -
Guntaka Ramareddy V.,
Gowda Siddareme,
Wagner Herbert,
Simon Dietrich
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80951-1
Subject(s) - enhancer , long terminal repeat , transcription (linguistics) , methylation , biology , terminal (telecommunication) , microbiology and biotechnology , genetics , transcription factor , gene , gene expression , computer science , computer network , linguistics , philosophy
The effect of methylation of an enhancer on transcription was studied. A 245 bp enhancer‐containing a fragment of the LTR of the avian sarcoma virus was methylated in vitro and ligated back into a vector which lacked the upstream enhancer sequence. The transient expression in QT6 cells indicated that methylation of the enhancer‐containing sequence severely reduced the extent of transcription.