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Gangliosides stimulate dome formation in cultured canine kidney epithelial cell line (MDCK)
Author(s) -
Rodrig N.,
Osanai T.,
Iwamori M.,
Nagai Y.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80947-x
Subject(s) - ganglioside , dome (geology) , sialic acid , kidney , chemistry , microbiology and biotechnology , cell culture , adenylate kinase , biochemistry , epithelium , biology , endocrinology , receptor , paleontology , genetics
The effect of exogenous gangliosides on the occurrence of domes in MDCK cell cultures was investigated in view of the involvement of both dome formation and gangliosides in cell growth, differentiation and transepithelial transport. Dome formation was increased by gangliosides in medium free of fetal calf serum. Among the gangliosides tested, GM3 and GD3 isolated from porcine kidney were most active, increasing the dome number 12–17‐fold. Since gangliosides from kidney were more active than those from brain and erythrocytes, the hydrophobic moiety as well as sialic acid might be involved in this activity. These results indicate that tissue‐specific molecules of gangliosides function as inducers or mediators of dome formation. The mechanism probably involves adenylate‐ cyclase or another transmembrane biosignal‐transducing system.