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Arachidonic acid‐induced release of calcium in permeabilized human neutrophils
Author(s) -
Beaumier L.,
Faucher N.,
Naccache P.H.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80942-0
Subject(s) - arachidonic acid , calcium , neutrophile , chemistry , neutrophil extracellular traps , biochemistry , immunology , biology , in vitro , inflammation , enzyme , organic chemistry
The addition of arachidonic acid to a suspension of digitonin‐permeabilized human neutrophils was found to induce, in a dose‐dependent manner (ED 50 about 15 μM), the release of calcium from internal stores. Arachidic acid was without effect, while linoleic acid and linolenic acid were (on a concentration basis) at least 5‐times less active than arachidonic acid. The activity of arachidonic acid appears to be due to the fatty acid itself and not to one of its metabolites. The pool of calcium mobilized by arachidonic acid includes that sensitive to inositol 1,4,5‐trisphosphate. These results demonstrate a significant intracellular role for arachidonic acid at the level of the internal mobilization of calcium in human neutrophils.