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8‐Bromoguanosine 3′:5′‐cyclic monophosphate decreases intracellular free calcium concentrations in cultured vascular smooth muscle cells from rat aorta
Author(s) -
Kai Hisashi,
Kanaide Hideo,
Matsumoto Takahiro,
Nakamura Motoomi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80941-9
Subject(s) - vascular smooth muscle , intracellular , vasodilation , endocrinology , calcium , medicine , depolarization , biophysics , chemistry , aorta , smooth muscle , calcium in biology , biology , biochemistry
The effects of 8‐bromoguanosine 3′:5′‐cyclic monophosphate (8‐Br cGMP) on intracellular free calcium concentrations ([Ca 2+ ] i ) in cultured rat aortic vascular smooth muscle cells (VSMCs) loaded with fura‐2 were recorded microfluorometrically. Irrespective of whether VSMCs were at rest (in 5 mM K + PSS), under Ca 2+ depletion (in Ca 2+ ‐free medium for 10 min) and K + depolarization (in high K + PSS), [Ca 2+ ] i was actively reduced and reached a new and lower steady‐state level with the application of 8‐Br cGMP. This may be the first and direct evidence that cGMP, a putative mediator of various vasodilators, actively reduces [Ca 2+ ] i in VSMCs.