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Effect of unbalanced diets on incorporation of δ‐aminolevulinic acid into cytochrome P‐450
Author(s) -
Amelizad Z.,
Narbonne J.F.,
Borin C.,
Robertson L.W.,
Periquet A.,
Oesch F.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80911-0
Subject(s) - cytochrome , microsome , casein , cytochrome c , chemistry , biochemistry , specific activity , hemeprotein , isozyme , cytochrome p450 , metabolism , chromatography , enzyme , heme , mitochondrion
The in vivo syntheses of two liver microsomal cytochromes P‐450 PB 3a , P‐450 UT 50 [(1987) Eur. J. Biochem., submitted] ( M r 50 000, 52 000) have been estimated by measuring the specific activity 2 h after i.p. administration of δ‐[ 3 H]aminolevulinic acid to male Sprague Dawley rats. The animals were fed either a standard rat chow (5% lard, 22% casein) or unbalanced diets (high lipid, 30% lard or low protein, 6% casein) with or without 50 ppm Phenoclor DP6. The high‐lipid diet supported a more rapid body weight gain but had little impact on cytochrome P‐450 content, expressed either per whole liver or per mg microsomal protein, and on the incorporation of the precursor into cytochrome P‐450. The latter was determined by measuring the radioactivity incorporated into the cytochrome P‐450 fraction, partially purified by afffinity chromatography, as well as into two cytochrome P‐450 isozymes ( M r 50 000 or 52 000) purified by DEAE‐52 cellulose ion‐exchange chromatography. The low‐protein diet, on the other hand, severely depressed body weight gain and cytochrome P‐450 content as well as incorporation of radioactivity, the lower‐ M r cytochrome ( M r 50 000) being particularly affected. However, when a potent inducer, Phenoclor DP6, was added to the low‐protein diet, cytochrome synthesis was restored indicating that the effect was reversible.