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Location of penicilloyl groups on CNBr fragments of the albumin from penicillin‐treated patients
Author(s) -
Lafaye Pierre,
Lapresle Claude
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80904-3
Subject(s) - chemistry , methionine , penicillin , pronase , biochemistry , albumin , disulfide bond , cleavage (geology) , residue (chemistry) , trypsin , amino acid , antibiotics , biology , enzyme , paleontology , fracture (geology)
Two fixation sites for penicilloyl groups on human albumin were demonstrated. Using CNBr cleavage the first site was located between methionine 123 and methionine 297 and the second one between methionine 297 and the C‐terminal residue. In both cases, penicilloyl groups were unmasked by pronase degradation or disulfide bond reduction.