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The GATATC‐modification enzyme Eco RV is closely related to the GATC‐recognizing methyltransferases Dpn II and dam from E . coli and phage T 4
Author(s) -
Lauster Roland,
Kriebardis Anastasios,
Guschlbauer Wilhelm
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80897-9
Subject(s) - methyltransferase , enzyme , genetics , chemistry , escherichia coli , biochemistry , biology , dna , methylation , gene
The amino acid sequence of Eco RV DNA methyltransferase which methylates the amino group of the 5′‐adenine residue of the target sequence GATATC has been found to be closely related to that of three other adenine methyltransferases, Dpn II, dam and dam T 4 , the target sequence of which is GATC. Despite large differences on the DNA level, the four sequences show four blocks of homologies. One of these blocks has the sequence DVYXDPPY and is found with little modification in numerous other DNA inethyltransferases. It is speculated that it could be the binding site of the methyl donor, S ‐adenosylmethionine. On the other hand, the identification of a DNA‐binding region is more tenuous. As expected, no analogies with (dimeric) repressors and cro proteins which have the characteristic helix‐turn‐helix motif have been observed.