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Triiodothyronine stimulates proliferation of osteoblast‐like cells in serum‐free culture
Author(s) -
Ernst M.,
Froesch E.R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80896-7
Subject(s) - osteoblast , triiodothyronine , endocrinology , alkaline phosphatase , medicine , cell growth , chemistry , matrix (chemical analysis) , biology , microbiology and biotechnology , biochemistry , in vitro , hormone , enzyme , chromatography
Primary rat ostcoblast‐like cells (Ob) were grown under strictly serum‐free conditions for up to 20 days. In the presence of triiodothyronine (T 3 ) at concentrations of 0.01 and 0.1 nM, Ob proliferation was enhanced. Moreover, a decrease of alkaline phosphatase (AP) activity, a differentiation marker for Ob, was prevented, whereas protein synthesis (collagen and noncollagen protein) was decreased. T 3 at much higher concentration (10 nM) had no significant effect on cell proliferation and matrix formation but decreased AP activity disproportionately. Thus, T 3 at close to physiological concentrations stimulates growth and maintains differentiation of Ob.