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Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli
Author(s) -
Maruyama Ikuo,
Oyamada Hideto,
Hasegawa Tomohiko,
Ohtsuka Kazuko,
Momose Kazutaka
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80881-5
Subject(s) - taenia coli , guinea pig , smooth muscle , intracellular , chemistry , cholinesterase , biochemistry , taenia , diisopropyl fluorophosphate , serine protease , microbiology and biotechnology , protease , biophysics , biology , pharmacology , endocrinology , enzyme , helminths , immunology
The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated spectrofluorometrically. The presence of DFP during the loading permitted the incorporation of quin 2 into the cells, so that it became possible to measure intracellular Ca 2+ concentrations using the ester of this dye. Also, DFP significantly enhanced the incorporation of fura 2 into the cells. These results indicate that loading of quin 2/AM and fura 2/AM into the smooth muscle cells may depend on the suppression of ChE or various serine protease activities outside cells.