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Splicing of group II introns in mRNAs coding for cytochrome b 6 and subunit IV in the liverwort Marchantia polymorpha chloroplast genome Exon specifying a region coding for two genes with the spacer region
Author(s) -
Fukuzawa Hideya,
Yoshida Tadashi,
Kohchi Takayuki,
Okumura Takeshi,
Sawano Yasuji,
Ohyama Kanji
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80876-1
Subject(s) - intron , gene , biology , group ii intron , marchantia polymorpha , rna splicing , genetics , exon , genome , primer extension , coding region , protein subunit , microbiology and biotechnology , rna
Two genes, petB and petD , encoding cytochrome b 6 and subunit IV of the cytochrome b 6 /ƒ complex, respectively, of the chloroplast genome of Marchantia polymorpha , a liverwort, have been mapped between the psbH and rpoA genes and each of the petB and petD genes has been predicted to contain a group II intron in its coding sequence [(1986) Nature 322, 572–574]. Primer extension analysis of the liverwort chloroplast RNA using two synthetic oligodeoxyribonucleotides, complementary to parts of the coding sequences of the petB and petD transcripts, as primers has provided evidence that the two genes are co‐transcribed to a precursor mRNA which is then precisely spliced at the predicted sites.