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Cyclic AMP induces a transient alkalinization in Dictyostelium
Author(s) -
Aerts Rob J.,
De Wit René J.W.,
Van Lookeren Campagne Michiel M.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80848-7
Subject(s) - dictyostelium discoideum , stimulation , stimulus (psychology) , chemotaxis , cytoplasm , biophysics , dictyostelium , chemistry , microbiology and biotechnology , biology , biochemistry , endocrinology , gene , receptor , psychotherapist , psychology
In a wide range of cell types, stimulus‐response coupling is accompanied by a rise in cytoplasmic pH (pH i ). It is shown that stimulation of developing Dictyostelium discoideum cells with the chemoattractant cAMP also results in a rise in pH i . About 1.5 min after stimulation, pH i starts increasing from pH i ∼7.45 to pH i ∼7.60, as is revealed independently by two different pH null‐point methods. The rise in pH i is transient, also with a persistent stimulus, and effectively inhibited by diethylstilbestrol (DES), strongly suggesting that the rise in pH i is accomplished by the DES‐sensitive plasma membrane proton pump which has been demonstrated in D. discoideum .