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A novel cholera toxin‐sensitive G‐protein (G c ) regulating receptor‐mediated phosphoinositide signalling in human pituitary clonal cells
Author(s) -
Lo William W.Y.,
Hughes John
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80840-2
Subject(s) - cholera toxin , pertussis toxin , g protein , inositol phosphate , muscarinic acetylcholine receptor , receptor , biology , toxin , microbiology and biotechnology , medicine , endocrinology , biochemistry , inositol
Recent studies have implicated that a GTP‐binding protein (G‐protein) is involved in the coupling of both CCK‐8 and muscarinic cholinergic receptors to phosphoinositidase C (PIC) in the human embryonic pituitary cell line, Flow 9000. Pretreatment of these cells with cholera toxin, but not pertussis toxin, inhibited the stimulation of [ 3 H]inositol phosphate production by CCK‐8 and acetylcholine. These inhibitory effects of cholera toxin could not be reproduced by treating the cells with the B‐subunit of cholera toxin or cAMP‐generating agents such as forskolin. These data suggest the presence of a novel G c protein which is responsible for receptor‐PIC coupling in Flow 9000 cells.