Premium
Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus
Author(s) -
Trakhanov S.D.,
Yusupov M.M.,
Agalarov S.C.,
Garber M.B.,
Ryazantsev S.N.,
Tischenko S.V.,
Shirokov V.A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80838-4
Subject(s) - thermus thermophilus , ribosome , thermophile , crystallization , ribosomal rna , crystallography , electron microscope , chemistry , ribosomal protein , bacteria , escherichia coli , 50s , biology , biochemistry , enzyme , genetics , physics , organic chemistry , rna , optics , gene
Well‐ordered three‐dimensional crystals of 70 S ribosomes and 30 S ribosomal subunits from extremely thermophilic bacteria Thermus thermophilus have been obtained. Positively stained thin sections of the crystals have been analyzed by electron microscopy. Redissolved crystalline ribosomes and small ribosomal subunits reveal sedimentation constants of 70 S and 30 S, respectively, and are functionally active in the poly(U)‐system.