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The influence of coupling transferrin to liposomes or minibeads on its uptake and fate in leukemic L 2 C cells
Author(s) -
Vidal M.,
Sainte-Marie J.,
Philippot J.R.,
Bienvenue A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80776-7
Subject(s) - transferrin , liposome , chemistry , coupling (piping) , biophysics , biochemistry , biology , materials science , metallurgy
Coupling transferrin to liposomes or minibeads did not affect its uptake by L 2 C lymphocytes via the Tf specific receptors. The uptake kinetics of Tf conjugated with particles about 50 nm in diameter was as rapid as in the case of native Tf, and the receptors were recycled with a similar turnover time (about 15 min). Contrary to the generally accepted scheme, we found some Tf degradation provoked by cellular uptake. The degradation represented about 10% of the amount of ligand taken up by the cells. It occurred when transferrin was coupled to liposomes, but not when coupled to minibeads.