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Regional distribution of leukotriene and mono‐hydroxyeicosatetraenoic acid production in the rat brain Highest leukotriene C 4 formation in the hypothalamus
Author(s) -
Miyamoto Tsumoru,
Lindgren Jan Åke,
Hökfelt Tomas,
Samuelsson Bengt
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80769-x
Subject(s) - leukotriene c4 , leukotriene , hypothalamus , hydroxyeicosatetraenoic acid , arachidonic acid , endocrinology , chemistry , medicine , lipoxygenase , leukotriene e4 , biochemistry , biology , enzyme , asthma
The regional distribution of ionophore A23187‐induced synthesis of leukotrienes and mono‐hydroxyeicosatetraenoic acids in the rat brain in vitro was investigated. Pronounced differences in leukotriene C 4 formation were observed, with the highest synthetic capacity in the hypothalamus. The formation of leukotriene C 4 was about 12‐times higher in the hypothalamus as compared to the cerebellum. This finding is in agreement with a possible neuroendocrine role for leukotriene C 4 . In contrast, the activity of leukotriene B 4 synthesis was widely distributed without pronounced regional differences in the rat brain. Formation of 5‐, 9‐, 11‐, 12‐ and 15‐monohydroxyeicosatetraenoic acid was detected in all regions. The major lipoxygenase product in the hypothalamus and thalamus was 5‐hydroxyeicosatetraenoic acid, while other monohydroxyeicosatetraenoic acids predominated in the remaining regions tested.