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Role of acidic phosphoproteins in the partial reconstitution of the active 60 S ribosomal subunit
Author(s) -
Lavergne Jean-Pierre,
Conquet François,
Reboud Jean-Paul,
Reboud Anne-Marie
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80761-5
Subject(s) - protein subunit , chemistry , ribosomal rna , biochemistry , gene
We have recently shown that rat liver 60 S ribosomal subunits active in protein synthesis can be reconstituted from inactive core particles lacking 30% of the total proteins, mainly L10a, L12, L22, L24, A33 and the acidic phosphoproteins P1–P2, obtained by treatment of 60 S subunits with dimethylmaleic anhydride [(1987) Eur. J. Biochem. 163, 15–20]. In this study, an ethanol extract of the 60 S subunit which contains only P1–P2 was also shown to be effective in reconstitution with the DMMA‐core‐particies: it strongly stimulated the EF‐2‐dependent GTP hydrolysis and, to a lesser extent, polyphenylalanine synthesis; like the DMMA wash it shifted the thermal denaturation curve of the DMMA‐core particles towards that of control subunits. Prior dephosphorylation of the ethanol extract by alkaline phosphatase inhibited the reconstruction process.