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Expression of ricin A chain in Escherichia coli
Author(s) -
O'Hare Mary,
Roberts Lynne M.,
Thorpe Philip E.,
Watson Graham J.,
Prior Bernadette,
Lord J.Michael
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80759-7
Subject(s) - ricin , escherichia coli , complementary dna , recombinant dna , plasmid , microbiology and biotechnology , expression vector , transcription (linguistics) , biology , dna , chemistry , gene , biochemistry , toxin , linguistics , philosophy
DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P N25 fused with the lac operator of E.coli . When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.