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In vivo enzymology: 13 C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b
Author(s) -
Mason Ralph P.,
Sanders Jeremy K.M.,
Cornish Alex
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80745-7
Subject(s) - isotopomers , chemistry , methanol , kinetic isotope effect , nuclear magnetic resonance spectroscopy , isotope , in vivo , analytical chemistry (journal) , radiochemistry , nuclear chemistry , stereochemistry , deuterium , organic chemistry , molecule , physics , microbiology and biotechnology , quantum mechanics , biology
The competitive oxidation of 13 CH 3 OH and 13 CD 3 OH has been observed using in vivo 13 C NMR spectroscopy. Simultaneous 1 H and 2 H decoupling gave isotopically shifted 13 C singlets for the two methanol isotopomers. The measured enzymic isotope effect, k H/ k D is approx. 1.8, indicating that CH bond cleavage is not rate‐determining.