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ADP‐ribosylation of specific membrane proteins in pheochromocytoma and primary‐cultured brain cells by botulinum neurotoxins type C and D
Author(s) -
Matsuoka Ichiro,
Syuto Bunei,
Kurihara Kenzo,
Kubo Shuichiro
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80709-3
Subject(s) - adp ribosylation , gtp' , pheochromocytoma , clostridium botulinum , membrane , chemistry , membrane protein , biochemistry , cell culture , adp ribosylation factor , cell , biology , microbiology and biotechnology , toxin , enzyme , endocrinology , nad+ kinase , genetics , golgi apparatus
Type C 1 and D toxins produced by Clostridium botulinum caused ADP‐ribosylation of a protein of 24 kDa in membrane preparations of rat clonal pheochromocytoma cells (PC12) and of proteins of 25 and 26 kDa in neuron‐rich culture of fetal rat brain cells. The ADP‐ribosylation reaction was dependent on the presence of MgCl 2 , GTP and GTPγS. The results obtained suggested that the ADP‐ribosylation reaction is responsible for the development of the biological activity of the botulinum neurotoxins and that the target of this reaction may be novel GTP‐binding proteins localized on cell membranes.