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Intracellular organelle motility and membrane fusion processes in human neutrophils upon cell activation
Author(s) -
Mollinedo Faustino,
Schneider Donald L.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80655-5
Subject(s) - degranulation , respiratory burst , granule (geology) , cytochalasin b , superoxide , microbiology and biotechnology , intracellular , chemistry , cytochalasin , motility , granulocyte , biochemistry , cell , biology , immunology , cytoskeleton , enzyme , paleontology , receptor
Release subcellular fractionation experiments indicate that fusion of a novel tertiary granule with the plasma membrane is concomitant with human neutrophil activation. Phorbol 12‐myristate 13‐acetate (PMA) induced a respiratory burst in human neutrophils as well as a high release of gelatinase, a marker of the tertiary granule. Preincubation of neutrophils with cytochalasin E induced a partially activated or ‘primed’ state, in which cells were unable to generate superoxide anion, but showed a reduced latency period for this activity. Fusion of tertiary granules with the cell surface also occurred during priming, although to a lesser extent than in PMA stimulation. The rapid tertiary granule degranulation, preceding that of specifics azurophilics, seems to play an important role in the functionality and secretory properties of human neutrophils.