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Identification of two forms of 6‐phosphofructo‐2‐kinase in yeast
Author(s) -
Aragón Juan J.,
Gómez María-Esther,
Gancedo Carlos
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80563-x
Subject(s) - yeast , identification (biology) , chemistry , biochemistry , computational biology , biology , botany
Two forms of 6‐phosphofructo‐2‐kinase have been identified in Saccharomyces cerevisiae by their different chromatographic behaviour on CM‐Sephadex C‐50. One of them was not adsorbed and represented ∼ 30% of the eluted activity. The other one emerged at about 120 mM KCl. A molecular mass of 120 kDa was found for both of them. No differences in kinetic behaviour in susceptibility to activation by cAMP‐dependent protein kinase were found between the two forms.