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The DNase I generated disomal series is coherent to 16 N Implications for coiling models of chromatin structure
Author(s) -
Davis S.J.,
Burgoyne L.A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80556-2
Subject(s) - chromatin , series (stratigraphy) , dna , chemistry , biology , biophysics , computer science , genetics , paleontology
The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16 N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple “back‐to‐back” double‐stranded structures within chromatin.