Premium
Triton X‐100 reacts with chlorophyll in the presence of chlorophyllase
Author(s) -
Michalski T.J.,
Bradshaw C.,
Hunt J.E.,
Norris J.R.,
Katz J.J.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80553-7
Subject(s) - transesterification , triton x 100 , chemistry , chlorophyll , hydrolysis , substrate (aquarium) , pulmonary surfactant , enzyme , chlorophyll a , chromatography , catalysis , organic chemistry , biochemistry , biology , ecology
Chlorophyllase (chlorophyll chlorophyllidohydrolase, EC 3.1.1.14) catalyses the transesterification of chlorophylls with the surfactant Triton X‐100, which is widely used in the preparation and study of this enzyme. The preparation and some properties of water‐soluble tritonyl chlorophyllide esters are described. A mechanism for the role of Triton X‐100 as an inhibitor in chlorophyllase‐catalyzed hydrolysis and transesterification of chlorophylls is proposed. Bacteriochlorophyl a also has been employed as a substrate for green plant chlorophyllase.