Premium
Complete amino acid sequence of poplar plastocyanin b
Author(s) -
Dimitrov Mitko I.,
Egorov Caesii A.,
Donchev Anthony A.,
Atanasov Boris P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80542-2
Subject(s) - plastocyanin , sequence (biology) , chemistry , amino acid , peptide sequence , polypeptide chain , amino acid residue , stereochemistry , biochemistry , chloroplast , gene , photosystem i
A second type of plastocyanin, plastocyanin b (PC b ) was isolated from leaves of poplar Populus nigra var. Italica . The complete amino acid sequence of 99 residues in the single polypeptide chain of plastocyanin b has been determined: DVLLGADDG SLAFVPSEFS GEKIVFK NNAGFPHN FDED PSGV D SKISMSEE DLLNAKGETF EVALS KGEY FYCSPHQGA GMVGKV VN The sequence obviously demonstrates, that PC b , in comparison with the known plastocyanin, plastocyanin a (PC a ), has 12 amino acid replacements (underlined letters): Ile 1→Val, Ile 21‐Ser 22‐Pro 23→Val‐Pro‐Ala, Ile 39‐Val 40→Val‐Leu, Ser 45‐Ile 46→ Ala‐Val, Ala 52→Val, Asn 76→Asp, Ser 81→Thr and Thr 97→Ile. The replacements at positions 40 and 76 of PC b are probably essential for differences in its redox and electrochemical properties, respectively.