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Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization
Author(s) -
Batenburg A.M.,
van Esch J.H.,
Leunissen-Bijvelt J.,
Verkleij A.J.,
de Kruijff B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80526-4
Subject(s) - melittin , chemistry , biochemistry , biophysics , biology , peptide
A characterization of the structural alterations induced by melittin in model‐membranes of dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on the use of 31 P‐NMR, freeze‐fracture electron microscopy and small angle X‐ray scattering. In accordance with earlier findings on the cardiolipinmelittin system, melittin is found to have an inverted phase inducing effect on these negatively charged lipids, in contrast to the influence on zwitterionic phospholipids. In phosphatidic acid this is expressed in the formation of an H II phase; in phosphatidylglycerol a less ordered, non‐lamellar structure with low water content is adopted.