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Regulation and specificity of glucose‐stimulated insulin gene expression in human islets of Langerhans
Author(s) -
Hammonds Peter,
Schofield Paul N.,
Ashcroft Stephen J.H.,
Sutton Robert,
Gray Derek W.R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80523-9
Subject(s) - islet , insulin , endocrinology , medicine , messenger rna , stimulation , extracellular , gene expression , biology , beta cell , blood sugar regulation , secretion , insulin oscillation , kinase , gene , microbiology and biotechnology , biochemistry
The insulin response of cultured human islets of Langerhans was measured at both mRNA and polypeptide levels in response to natural and pharmacological stimuli. We report a dosage dependent stimulation of both mRNA levels and insulin secretion by extracellular glucose, and present evidence that islet responsiveness can be divided into two temporal phases: an early response, apparently under post‐transcriptional control, and a late phase in which insulin messenger accumulates. Although glucose effects in man are similar to rodents, there are important differences, especially with respect to modulation of glucose stimulation by activators of β‐cell protein kinases.