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Heat shock inactivates a supernatant factor(s) specifically required for efficient expression of the amp gene in Escherichia coli
Author(s) -
Kuriki Yoshitaka
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80522-7
Subject(s) - escherichia coli , pbr322 , gene expression , microbiology and biotechnology , gene , in vitro , chemistry , psychological repression , biology , biochemistry
A 160 000 × g supernatant of E. coli extract prepared from cells grown at 30°C stimulated specifically the expression of the amp gene on pBR322 and pBR328 in an in vitro gene expression system from E. coli . This activity of the supernatant was markedly reduced when the cells were exposed to 42°C for 30 min prior to preparing the supernatant. These results are consistent with the view that heat shock‐induced repression of the amp gene expression is due to inactivation of a supernatant factor(s) required for effective expression of the amp gene.