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Expression of β‐nerve growth factor mRNA in rat glioma cells and astrocytes from rat brain
Author(s) -
Yamakuni Tohru,
Ozawa Fumiko,
Hishinuma Fumio,
Kuwano Ryozo,
Takahashi Yasuo,
Amano Takehiko
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80520-3
Subject(s) - nerve growth factor , northern blot , messenger rna , microbiology and biotechnology , biology , gene expression , astrocyte , western blot , gene , southern blot , central nervous system , endocrinology , receptor , biochemistry
A 50‐base synthetic oligodeoxynucleotide complementary to a portion of mouse nerve growth factor (NGF) mRNA was used as a probe for analysis of the expression of NGF gene. Northern blot analysis showed the presence of a major 1.3 kb transcript, which was identical in size to mouse NGF mRNA, in both C6Bu1 cells and rat astrocytes cultured from newborn rat brain. Further, the rearrangement of DNA sequence in and around the NGF gene locus of C6Bu1 cells was not detected by Southern blot analysis. These results indicate the expression of NGF mRNA in both C6Bu1 cells and astrocytes from rat brain, suggesting that astrocytes may produce NGF protein in the rat brain, especially in developing rat brain.