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Vertical fluctuations of phospholipid acyl chains in bilayers
Author(s) -
Wardlaw John R.,
Sawyer William H.,
Ghiggino Kenneth P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80502-1
Subject(s) - quenching (fluorescence) , phospholipid , fluorophore , chemistry , vesicle , fluorescence , lipid bilayer , palmitic acid , biophysics , membrane , photochemistry , organic chemistry , biochemistry , fatty acid , physics , optics , biology
The possibility of vertical displacement of acyl chains in lipid bilayers has been examined by quenching the fluorescence of 2‐(9‐anthroyloxy)palmitic acid with 5‐ and 16‐doxylstearates in dipalmitoylphosphatidyl‐choline unilamellar vesicles. Measurement of lifetime and steady‐state quenching showed that the dynamic component of quenching was independent of the transverse position of the quencher indicating that a quencher at the 16‐position could interact with a fluorophore at the 2‐position with high frequency. The differences in steady‐state quenching could be accounted for by the differences in the static component of quenching. The results provide further evidence for rapid vertical displacements of acyl chains in phospholipid bilayers.