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Two major tyrosine protein kinases of resting human T lymphocytes are down‐regulated following mitotic stimulation
Author(s) -
Hall Belinda S.,
Hoffbrand A.Victor,
Wickremasinghe R.Gitendra
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80499-4
Subject(s) - phytohaemagglutinin , tyrosine , biology , size exclusion chromatography , biochemistry , lymphocyte , mitosis , stimulation , microbiology and biotechnology , immunology , endocrinology , enzyme
Human lymphocyte tyrosine protein kinases (TPKs) have been analyzed by gel‐filtration chromatography. The major TPK species with activity towards an exogenous tyrosine‐containing peptide had molecular masses of 70–100 kDa (TPK I) and 35–40 kDa (TPK II). TPKs I and II were distinct from the well‐characterized autophosphorylating lymphoid cell TPK, pp56 lck [(1983) J. Biol. Chem. 258, 10738–107421]. Both TPK I and TPK II were down‐regulated following mitogenic stimulation of lymphocytes with phytohae‐magglutinin. By contrast, pp56 lck remained clearly detectable in stimulated lymphocytes. We suggest that TPKs I and II may play a role in the regulation of the lymphocyte cell cycle.