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Identification of the lesion in the stimulatory GTP‐binding protein of the uncoupled S49 lymphoma
Author(s) -
Rall Thomas,
Harris Bruce A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80486-6
Subject(s) - protein subunit , transversion , g alpha subunit , mutant , g protein , receptor , gi alpha subunit , gtp' , interleukin 10 receptor, alpha subunit , gtp binding protein regulators , biology , cyclase , microbiology and biotechnology , chemistry , biochemistry , mutation , gene , enzyme
The stimulatory GTP‐binding protein (G s ) of the uncoupled mutant of S49 lymphoma cells is deficient in its ability to transduce hormonal signals from ligand‐bound β‐adrenergic receptors to the catalytic component of adenylate cyclase. In order to define the genetic defect in the G s of uncoupled S49 cells, a complementary DNA clone encoding the α‐subunit of G s was analyzed and the deduced primary structure of the defective subunit compared to that of the wild‐type subunit. A single nucleotide transversion was found that coded for a proline rather than an arginine at residue 389. The results indicate a domain of the α‐subunit of G s that specifically interacts with hormone receptors.