Premium
Isolation of two different subpopulations of the light‐harvesting chlorophyll a/b complex of photosystem II (LHCII)
Author(s) -
Spangfort Michael,
Larsson Ulla K.,
Anderson Jan M.,
Andersson Bertil
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80481-7
Subject(s) - spinach , photosystem ii , chlorophyll , biology , isoelectric focusing , chlorophyll a , isoelectric point , chemistry , biochemistry , photosynthesis , botany , enzyme
Isolated LHCII from spinach has been solubilized and fractionated by non‐denaturing isoelectric focusing to yield two subpopulations with different polypeptide but equal chlorophyll composition. One LHCII subpopulation contains only a 27 kDa polypeptide while the other contains the 27 and 25 kDa polypeptides in about equal amounts. The polypeptide patterns of the two subpopulations closely correspond to those suggested previously for the inner LHCII and peripheral LHCII, respectively.