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Effect of phase transitions in hydrated 1,2‐dipalmitoylphosphatidylethanolamine bilayers on the spin probe order parameter
Author(s) -
Horniak L.,
Kutejová E.,
Balgavý P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80470-2
Subject(s) - spin probe , chemistry , spin label , phase transition , bromide , phase (matter) , site directed spin labeling , crystallography , analytical chemistry (journal) , nuclear magnetic resonance , membrane , inorganic chemistry , chromatography , thermodynamics , organic chemistry , physics , biochemistry
The ‘main’ phase transition L β →L α of hydrated 1,2‐dipalmitoylphosphatidylethanolamine (DPPE) bilayers in excess water affects the ESR order parameter S 33 of N ‐cetyl‐ N,N ‐dimethyl‐ N ‐tempoylammonium bromide (CAT‐16), 5‐doxylstearic acid (5‐DSA) and 16‐doxylstearic acid (16‐DSA) spin probes. The ‘pretransition’ and ‘subtransition’ suggested to occur in hydrated DPPE by Chowdhry et al. [(1984) Biophys. J. 45, 901–904] and Silvius et al. [(1986) Biochemistry 25, 4249–4258], respectively, affect exclusively the S 33 of CAT‐16, but not that of 5‐DSA and 16‐DSA spin probes. The subtransition occurs about 15 ± 1°C below the main transition.