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A monoclonal antibody against a synthetic peptide reveals common structures among spectrins and α‐actinin
Author(s) -
Närvänen Outi,
Närvänen Ale,
Wasenius Veli-Matti,
Partanen Paul,
Virtanen Ismo
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80440-4
Subject(s) - monoclonal antibody , spectrin , peptide , biology , homology (biology) , biochemistry , microbiology and biotechnology , peptide sequence , antibody , genetics , gene , cell , cytoskeleton
A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken α‐fodrin repeats reacts in immunoblotting with avian α‐spectrin and α‐fodrin, both mammalian spectrins and with mammalian α‐fodrin. This Mab also reacts with α‐actinin in both chicken and human cells. Our results confirm the previously detected structural homology between spectrins and α‐actinin and implicate their common evolutionary origin.