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Primary structure of ovine pituitary basic fibroblast growth factor
Author(s) -
Simpson Richard J.,
Moritz Robert L.,
Lloyd Christopher J.,
Fabri Louis J.,
Nice Edouard C.,
Rubira Michael R.,
Burgess Antony W.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80435-0
Subject(s) - primary (astronomy) , fibroblast growth factor receptor 2 , fibroblast growth factor receptor 4 , fibroblast growth factor receptor 3 , fibroblast growth factor receptor , endocrinology , protein primary structure , fibroblast growth factor , chemistry , medicine , microbiology and biotechnology , biology , biochemistry , peptide sequence , receptor , physics , gene , astronomy
The complete amino acid sequence of basic FGF (146 residues) from ovine pituitary glands has been established. This has been achieved by the sequence analysis of subnanomole amounts of the intact molecule and of peptides derived by enzymatic digestions with clostripain, chymotrypsin, pepsin and Staphylococcus aureus V8 protease. Microbore HPLC, employing 1–2 mm i.d. columns, was used to purify, concentrate and buffer‐exchange the FGF peptides. A novel application of ion‐pairing chromatography was employed to isolate peptides which were not retained on conventional reversed‐phase systems. There is only one positional difference between the ovine and bovine basic FGF s , but there are 3 positional differences between ovine and human basic FGFS.