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Eukaryotic elongation factor Tu is present in mRNA‐protein complexes
Author(s) -
Greenberg Jay R.,
Slobin Lawrence I.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80421-0
Subject(s) - ef tu , elongation factor , elongation , messenger rna , chemistry , microbiology and biotechnology , biology , biochemistry , gene , rna , translation (biology) , ribosome , materials science , ultimate tensile strength , metallurgy
By two‐dimensional gel electrophoresis, partial peptide mapping, and antibody binding we have shown that eukaryotic elongation factor Tu is in close contact with mRNA in rabbit reticulocytes. It can be crosslinked to mRNA by irradiating both polysomes and 40–80 S mRNA‐protein complexes with short‐wave UV light. To our knowledge this is the first case in which a known translation factor has been shown to be associated with mRNA in native ribonucleoproteins.