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Differential expression of α‐subunits of G‐proteins in human neuroblastoma‐derived cell clones
Author(s) -
Klinz Franz-Josef,
Yu Victor C.,
Sadée Wolfgang,
Costa Tommaso
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80419-2
Subject(s) - neuroblastoma , differential (mechanical device) , protein subunit , biology , microbiology and biotechnology , genetics , cell culture , gene , physics , thermodynamics
The distribution of α‐ and β‐subunits of G‐proteins was analyzed in membranes of three cell clones which are derived from the human neuroblastoma cell line SK‐N‐SH. The neuroblast‐like clone SH‐SY5Y shows a pattern of G‐proteins very similar to that of human brain cortex with high levels of G iα and G oα but low levels of G 40α . The intermediate clone SH‐IN contains high levels of G oα and G iα and moderate levels of G 40α . The non‐neuronal clone SH‐EP shows high levels of G 40α but lacks G oα . Differentiation of the neuroblast‐like clone SH‐SY5Y by retinoic acid or nerve growth factor does not change the amount of G iα or G oα in the membrane.