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Different abilities of two distinct Fcγ receptors on guinea pig polymorphonuclear leukocytes to trigger the arachidonic acid metabolic cascade
Author(s) -
Sato Masaki,
Nakamura Tohoru,
Koyama Jiro
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80416-7
Subject(s) - arachidonic acid , guinea pig , receptor , chemistry , biochemistry , immunology , biology , endocrinology , enzyme
The nature of signals transmitted by two Fcγ receptors (Fcγ 2 R for IgG2 and Fcγ 1 /γ 2 R for both IgG2 and IgG1) on guinea pig polymorphonuclear leukocytes was investigated. The specific binding of hen ovalbumin (OA)‐complexed IgG1 antibody to Fcγ 1 /γ 2 R did not seem to trigger the release of [ 3 H]arachidonic acid from the cells prelabeled with [ 3 H]arachidonic acid. In contrast, marked release occurred on the binding of OA‐complexed IgG2 antibody to the cells. This reaction was not inhibited, but rather enhanced, by the Fab′ of a monoclonal antibody to Fcγ/γ 2 R. Therefore, a signal for the activation of the arachidonic acid metabolic cascade was found to be transmitted by Fcγ 2 R, but scarcely by Fcγ 1 /γ 2 R.