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Ca 2+ uptake and IP 3 ‐induced Ca 2+ release in permeabilized human lymphocytes
Author(s) -
Eberl G.,
Schnell K.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80400-3
Subject(s) - chemistry , radiochemistry , biophysics , biology
The 45 Ca 2+ uptake and 45 Ca 2+ release in saponin‐permeabilized human lymphocytes were studied. An ATP‐dependent Ca 2+ uptake into a nonmitochondrial, intracellular Ca 2+ store is observed which is approx. 2 orders of magnitude greater than the ATP‐independent Ca 2+ uptake. The Ca 2+ uptake is inhibited by vanadate, but it is insensitive to oligomycin and ruthenium red. IP 3 induces dose‐dependent 45 Ca 2+ release. For half‐maximum Ca 2+ release 0.25–0.5 μM IP 3 is required. The results of our studies suggest that 45 Ca 2+ is predominantly stored within the endoplasmic reticulum of the lymphocytes.