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Pertussis toxin does not affect the time course of exocytosis in mast cells stimulated by intracellular application of GTP‐γ‐S
Author(s) -
Lindau Manfred,
Nüße Oliver
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80393-9
Subject(s) - exocytosis , pertussis toxin , secretion , gtp' , stimulation , g protein , intracellular , microbiology and biotechnology , chemistry , mast cell , receptor , biology , biophysics , endocrinology , biochemistry , immunology , enzyme
Exocytosis was studied in single rat peritoneal mast cells. Granule fusion was monitored by time‐resolved capacitance measurements using the patch‐clamp technique. Intracellular stimulation of mast cells with 20 μM GTP‐γ‐S stimulates exocytosis with a calcium‐dependent time course. Secretion in response to receptormediated stimulation with compound 48/80 was completely abolished by treatment with pertussis toxin (IAP) at 180 ng/ml for 4 h. The time course of exocytosis in response to GTP‐γ‐S remained unaffected in IAP‐treated cells supporting the involvement of a second GTP‐binding protein in stimulus‐secretion coupling.