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Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells Effects of cell density
Author(s) -
Lomri Abderrahim,
Marie Pierre J.,
Escurat Michel,
Portier Marie-Madeleine
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80392-7
Subject(s) - cytoskeleton , vimentin , actin , methionine , gel electrophoresis , protein biosynthesis , intermediate filament , polyacrylamide gel electrophoresis , microbiology and biotechnology , chemistry , cell , biochemistry , biology , enzyme , amino acid , immunology , immunohistochemistry
The most abundant cytoskeletal proteins synthesized in mouse endosteal osteoblastic cells were identified employing two‐dimensional polyacrylamide gel electrophoresis and immunoblotting. The relative rate of synthesis of the proteins were measured on radioautograms of detergent‐soluble and ‐insoluble lysates of the cells labeled with [ 35 S]methionine. Doubling initial cell density induced a 10–45% reduction in the de novo synthesis of actin, α‐actinin, vimentin and β‐tubulins with no change in α‐tubulins. Increasing cell density caused a 45% decrease in the polymerized form of actin with no change in the unpolymerized fraction, suggesting a correlation of alteration of the organization and synthesis of proteins.